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(A, B) 2D (A) and 3D (B) SIM imaging of Halo-KIF13B (red) and IFT172-eGFP (green) stably expressed <t>in</t> <t>hTERT-RPE1</t> cells subjected to 24 hours of starvation. In panel B, the white line was used to calculate fluorescence intensity profiles. Scale bars are 2 µm. (C) Fluorescence intensity profile calculated for the same cilium as in (B), drawing a line near the ciliary base, perpendicular to the axoneme. (D) Confocal (right) and 2D STED (left) images of fixed hTERT-RPE1 cells expressing Halo-KIF13B (red) and IFT172-eGFP (gray) after 24 hours of starvation, with FBF-1 antibody staining (cyan). Here, a Gaussian blur with a sigma of 2 pixels is used to smooth the images. Scale bars are 1 µm.
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(A, B) 2D (A) and 3D (B) SIM imaging of Halo-KIF13B (red) and IFT172-eGFP (green) stably expressed <t>in</t> <t>hTERT-RPE1</t> cells subjected to 24 hours of starvation. In panel B, the white line was used to calculate fluorescence intensity profiles. Scale bars are 2 µm. (C) Fluorescence intensity profile calculated for the same cilium as in (B), drawing a line near the ciliary base, perpendicular to the axoneme. (D) Confocal (right) and 2D STED (left) images of fixed hTERT-RPE1 cells expressing Halo-KIF13B (red) and IFT172-eGFP (gray) after 24 hours of starvation, with FBF-1 antibody staining (cyan). Here, a Gaussian blur with a sigma of 2 pixels is used to smooth the images. Scale bars are 1 µm.
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(A, B) 2D (A) and 3D (B) SIM imaging of Halo-KIF13B (red) and IFT172-eGFP (green) stably expressed in hTERT-RPE1 cells subjected to 24 hours of starvation. In panel B, the white line was used to calculate fluorescence intensity profiles. Scale bars are 2 µm. (C) Fluorescence intensity profile calculated for the same cilium as in (B), drawing a line near the ciliary base, perpendicular to the axoneme. (D) Confocal (right) and 2D STED (left) images of fixed hTERT-RPE1 cells expressing Halo-KIF13B (red) and IFT172-eGFP (gray) after 24 hours of starvation, with FBF-1 antibody staining (cyan). Here, a Gaussian blur with a sigma of 2 pixels is used to smooth the images. Scale bars are 1 µm.

Journal: bioRxiv

Article Title: Analysis of motor-based transport in primary cilia by dynamic mode decomposition of live-cell imaging data

doi: 10.64898/2026.03.27.714708

Figure Lengend Snippet: (A, B) 2D (A) and 3D (B) SIM imaging of Halo-KIF13B (red) and IFT172-eGFP (green) stably expressed in hTERT-RPE1 cells subjected to 24 hours of starvation. In panel B, the white line was used to calculate fluorescence intensity profiles. Scale bars are 2 µm. (C) Fluorescence intensity profile calculated for the same cilium as in (B), drawing a line near the ciliary base, perpendicular to the axoneme. (D) Confocal (right) and 2D STED (left) images of fixed hTERT-RPE1 cells expressing Halo-KIF13B (red) and IFT172-eGFP (gray) after 24 hours of starvation, with FBF-1 antibody staining (cyan). Here, a Gaussian blur with a sigma of 2 pixels is used to smooth the images. Scale bars are 1 µm.

Article Snippet: The hTERT-RPE1 parental cell line stably expressing IFT172-eGFP (derived from the immortalized hTERT-RPE1 cell line, ATCC, clone CRL-4000) has been described previously ( ; ). hTERT-RPE1 stably co-expressing IFT172-eGFP and Halo-KIF13B were generated by transducing the aforementioned cell line with lentiviral particles expressing pCDH-EF1aGW-IRES-Blast-Halo-KIF13B plasmid.

Techniques: Imaging, Stable Transfection, Fluorescence, Expressing, Staining